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- W2384459597 abstract "Aim To establish a rapid,sensitive and specific diagnostic assay for scrub typhus with microarray techniques.Methods Firstly,the specific primers and probe were designed according to the conservative and specific DNA sequence of 56kD protein of orientia tsutsugamush.Scecondly,the probes were spotted on glass slides to form microarrays.Then,the Cy3-labled Ot DNA fragment prepared by dissymmetical PCR were hybridized to the microarrays.Finally,the microarrays were scanned and analyzed with a scanner.Results The DNA microaray could detect the DNA of Ot standard strains of scrub typhus with adequate specificity and sensitivity.Conclusion The successfully developed DNA microarray might be widely applied in specific detection of various Ot samples and be a very useful method for diagnosis of Scrub typhus." @default.
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- W2384459597 date "2003-01-01" @default.
- W2384459597 modified "2023-09-26" @default.
- W2384459597 title "Establishment of diagnostic assay for scrub typhus with microarray techniques" @default.
- W2384459597 hasPublicationYear "2003" @default.
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