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- W2384464697 abstract "A high performance liquid chromatographio (HPLC) method using fluorimeter as a detector with the excitation wavelength at 362 nm and the emission wavelength at 511 nm, was established for determining women's urinary estriol (E3), estradiol (E2) and estrone (E1) as their dansyl derivatives. This method comprises the following procedures: taking 1/1000 of 24-hour urine specimen, acid hydrolysis of the conjugates of estrogens, extraction of free estrogens with ether and alkline solution, preparation of dansyl derivatives of the estrogens and finally by HPLO determination. The chromatographic column was 150 mm × 4.6 mm (inside diameter) and slurry-packed with Qing-dao sillioa gel YWG-5 μm. Two solvent systems of different components and proportion were used as eluate for the determination of E3 and E2, E1. respectively. The eluate for determination of E3 was oyolohexane-ohloroform-triethylamine-isopropanol (5:95:10:12, v:v). Ez and E1 were determinated by gradient eluation with mobile phase A of cyclohexane-ohloroform-triethylamine--isopropanol (60:40:3:0.15, v: v) and mobile phase B of oyolohexane-triethylamine-isopropanol (100:3:0.15, v:v)The experimental results: the minimum detectable amount was about 0,1 ng; the values of coefficient of variation were Es4%, E26%, E17.5%; the correlation coefficient was r0.99; the values of extracting recovery were E3=83.9±3.8%, E2 = 74.7±4.6%, E1 = 73.4±4.7%.This method has the advantages of high sensitivity, small sampling amount and distinct separation, and is applicable to determining pregnancy or non-pregnancy urinary estrogens clinically." @default.
- W2384464697 created "2016-06-24" @default.
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- W2384464697 date "1982-01-01" @default.
- W2384464697 modified "2023-09-26" @default.
- W2384464697 title "HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF URINARY ESTROGENS" @default.
- W2384464697 hasPublicationYear "1982" @default.
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