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- W2384483010 abstract "Engineering bacteria with high density cultivation in Mycobacterium bovis DNA Vaccine were explored in this article.Three kinds of media were screened by the flask experiments,and a semi-synthetic medium which has the potential of high-density fermentation as the pilot scale medium was selected in the fermentor.The culturation temperature,dissolved oxygen,pH,rotational velocity,and feeding methods etc.were optimized,the E.coli density of fermentation is up to OD600 22.92 with the semi-synthetic medium,and the humid weight of engineering bacteria is 27.173 g/L.The outcome of plasmid is 1.44 mg/g.The stability of the plasmid in the engineering bacteria was also verification." @default.
- W2384483010 created "2016-06-24" @default.
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- W2384483010 date "2010-01-01" @default.
- W2384483010 modified "2023-09-28" @default.
- W2384483010 title "Study on the High Density Cultivation of Mycobacterium bovis DNA Vaccine Engineering Bacteria" @default.
- W2384483010 hasPublicationYear "2010" @default.
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