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- W2384550681 abstract "Follicular oocytes were recovered using three different methods(aspiration,dissection and puncturing).The average total number of oocytes recovered per ovary was significantly higher by the aspiration method.But the time required for processing each ovary and usable oocytes by the aspiration were comparatively more,so aspiration was not a good method to recover oocytes.The numbers of oocytes recovered per ovary were 5.6 and 6.7 for dissection and puncturing,respectively.The percentages of oocytes of A,B and C grade for dissection were 49 %,43 % and 8 %,and for aspiration were 52 %,42 % and 6 %,respectively.There were no significant differences between the two methods(P0.05).But time required by dissection was more than puncturing.Growth culture medium was supplemented with EGF of 20 ng/ml,30 ng/ml and 50 ng/ml.Compared with control group,EGF(30 ng/ml) significantly promoted maturation of the oocytes,but had no stimulatory effect on cleavage of the zygotes.EGF(50 ng/ml) extremely significantly promoted the maturation of the oocytes and the cleavage of the zygotes." @default.
- W2384550681 created "2016-06-24" @default.
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- W2384550681 date "2007-01-01" @default.
- W2384550681 modified "2023-09-24" @default.
- W2384550681 title "Effect of Recovery Methods and EGF on in Vitro Maturation of Goat Oocytes" @default.
- W2384550681 hasPublicationYear "2007" @default.
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