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- W2384588495 abstract "Objective To explore the significance and value of HBV pre-c region genes used in the early diagnosis of hepatitis B. Methods The partial gene sequence of HBV pre-c region in pBR322-HBV was amplified by PCR. The products amplified by PCR were sub-cloned into the pBS-T vector. They were an effective AT clone plasmid. The positive AT sub-clones were sequenced. Standard products were made and standard curve was obtained from real-time fluorescence quantitative PCR. Accuracy and repeatability were analyzed. Results The expected plasmid with HBV pre-c region gene was harvested. The correlation coefficient of standard curve for real-time fluorescence quantitative PCR was 0.995. The linear range was from 5×103 copies/μl to 5×109 copies/μl. All the coefficient of variation within batch and between batch were smaller than 5%. Both precision and repeatability were good. Conclusion HBV pre-c region genes to be used in the early detection of Hepatitis B are prospective. Standard curve of real-time fluorescence quantitative PCR for hepatitis B has been successfully made in this paper. Finding of this research has set a very good fundation for real-time fluorescence quantitative PCR applied in the field of clinical detection of hepatitis B." @default.
- W2384588495 created "2016-06-24" @default.
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- W2384588495 date "2007-01-01" @default.
- W2384588495 modified "2023-09-23" @default.
- W2384588495 title "Subcloning of partial sequence of gene of HBV pre-C region and preparation of standard curve for real-time flujorescence quantitiative PCR" @default.
- W2384588495 hasPublicationYear "2007" @default.
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