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- W2384598983 abstract "Objective:to isolate and identify esophageal carcinoma Eca109 stem cell.1) We bought Eca109 cell line from Shanghai Institute for Biological Sciences,performed monoclonal culture by the finite dilution method,and observed in HE staining;2) The surface antigens P75NTR and Oct-4 were detected by immunocytochemical technique in Eca109 monoclonal cell line;3) The cell proliferation condition was tested by MTT method and tablet cloning experiment;4) invasive ability of each cell line was detected by Transwell chamber assay.Two kinds of cellular morphologies were displayed in HE staining,one is round with large nucleus and the other is short shuttle-like with small nucleus.In Eca109 monoclonal cells,P75NTR has strong expression,Oct-4 expression is relatively weak,and the round cell line in expression is located in the nucleus.According to MTT results,the round cell line proliferation rate is obviously higher than the others,and the difference was statistically significant(P0.001).The round P75NTR and Oct-4 positive cells of Eca109 cells may be esophageal cancer stem cells." @default.
- W2384598983 created "2016-06-24" @default.
- W2384598983 creator A5072859995 @default.
- W2384598983 date "2013-01-01" @default.
- W2384598983 modified "2023-09-26" @default.
- W2384598983 title "Research into the Stem Cell Characteristics of Esophageal Carcinoma Eca109 Monoclonal Cell Line" @default.
- W2384598983 hasPublicationYear "2013" @default.
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