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- W2384604049 abstract "Based on nuclei polymorphism of fimH gene sequence of Salmonella pullorum at site 37 and 544 compared with Salmonella gallinarum from GenBank , a pair of allele-specific primers was designed , and an allele-specific polymerase chain reaction (AS-PCR) method for detecting S.pullorum was developed.Clinical isolated samples of S.pullorum were detected by PCR. The results showed that the method could specifically determine S.pullorum, and the PCR product was 543 bp. Sensitivity of PCR assay for DNA and pure cell culture of S.pullorum were 12.6 pg/μL and 2.3×104 cfu/mL , respectively. This PCR method was applicable for detecting S.pullorum in cloacal swabs , eggs , feed and drinking water. Therefore , it′s a easy , quick , specific and sensitive method , and could be applied for rapid detection of S. pullorum ." @default.
- W2384604049 created "2016-06-24" @default.
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- W2384604049 date "2013-01-01" @default.
- W2384604049 modified "2023-09-25" @default.
- W2384604049 title "Development and Application of Allele-specific PCR for Detection of Salmonella pullorum" @default.
- W2384604049 hasPublicationYear "2013" @default.
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