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- W2384610855 abstract "To explore the possibility of generation of transgenic chickens by direct microinjection of transgenes into the germinal disc of developing embryos. 1.5 kb human growth hormone (hGH) mini gene fused with goat β-lactoglobulin (BLG) gene promoter and human tissue kallikein-1 mixed with LipofectAMine reagent, were injected respectively into the germinal disc of one-day incubated chicken embryos. Among 320 microinjected embryos,194 and 108 embryos died during the first seven days and 8～18 days post incubation ,respectively and 18 chickens were hatched with a hatch percentage of 5.6%(18/320). Both dot blotting and Southern blotting analyses showed that the BLG-hGH construct was integrated into the genomes of 3 out of 44 embryos died during 4 to 18 days post incubation and that of 3 hatched chickens. The KLK1 construct was integrated into the genomes of 18 out of 94 embryos died during 4 to 18 days post incubation and that of 6 hatched chickens. These data further demonstrated that direct microinjection of foreign genes into the germinal disc of developing embryos was a useful way of generation transgenic chickens." @default.
- W2384610855 created "2016-06-24" @default.
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- W2384610855 date "2004-01-01" @default.
- W2384610855 modified "2023-09-23" @default.
- W2384610855 title "Transfect of Exogenous in Vivo of Chickens Germinal Disc" @default.
- W2384610855 hasPublicationYear "2004" @default.
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