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- W2384612842 abstract "ObjectiveTo obtain and analyze sequence EF-1 gene,and lay bases for screening candidate antigen of echinococcus granulosus.MethodsTotal RNA was extracted from protoscoles of cysts from humam.The specific primers were designed according to published nucletid sequence in the genbank database.The EF-1 gene of echinococcus granulosus was amplified by RT-PCR and cloned into pGEM-T vector for sequencing and analyzing.ResultsA cDNA sequence with an open reading frame of 693bp has been amplified successfully by RT-PCR.Comparision of the DNA and amino acid sequence deduced from cDNA with the published EF-1 gene sequence of echinococcus granulosus in the genbank revealed 99% identity samely.ConclusionBecause the EF-1 play an importante role in energy metabolism of E.granulosus,EF-1 gene gained from protoscoles can be used as candidate antigene gene to develope vaccine and its biological functions studying." @default.
- W2384612842 created "2016-06-24" @default.
- W2384612842 creator A5033854234 @default.
- W2384612842 date "2005-01-01" @default.
- W2384612842 modified "2023-09-24" @default.
- W2384612842 title "Cloning and sequence analyzing of the EF-1 gene from echinococcus granulosus of mankind" @default.
- W2384612842 hasPublicationYear "2005" @default.
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