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- W2384661350 abstract "AIM: To observe the differentiation and development of human mesenchymal stem cells (MSCs) transplanted onto corneal stroma of rabbits and investigate the feasibility of MSCs differentiated into corneal epithelium like cells.METHODS: 24 New Zealand albino rabbits were randomly divided into 2 groups: The human MSCs combined with amniotic membrane were transplanted onto the experimental animals, and the controls were transplanted with the amniotic membrane only. The MSCs were cultured on preserved human amniotic membrane for 4 days and labeled with 5'-Bromo-2'-deoxyuridine (BrdU), then the cells were transplanted onto the surface of the corneal stroma of the rabbits. The eyeballs were taken off after 1, 2, 3, 4, 6 and 8 weeks. The growth and differentiation of human MSCs were observed by histopathological and immunohistochemical examination.RESULTS: When the MSCs cultured on amniotic membrane were transplanted onto the surface of the corneal stroma of rabbits, the corneal epithelium were positive in CK3/CK12 staining and negative in CK 13 staining, revealed by immunohistochemical examination. The BrdU positive cells in the reconstructive corneal epithelium were found and showed positive in CK3/CK12 staining. CONCLUSION: After transplanted onto the corneal stroma of rabbits with human amniotic membrane, the MSCs survive, proliferate and differentiate into corneal epithelium like cells." @default.
- W2384661350 created "2016-06-24" @default.
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- W2384661350 date "2007-01-01" @default.
- W2384661350 modified "2023-09-27" @default.
- W2384661350 title "The preliminary study on differentiation of mesenchymal stem cells into corneal epithelium like cells" @default.
- W2384661350 hasPublicationYear "2007" @default.
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