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- W2384661422 abstract "The Gastrodia Antifungal Protein GAFP-I was purified from topical corm of Tian Ma( Gastrodia elata ), which can strongly inhibit the mycelia growth of seprophytic fungi and plays an important role in the defence mechanism of plants to restrict and prevent the infection of the fungus Armillaria mellea . This paper reports the primary structure and the cDNA nucleotide sequence of GAFP-I. The N-terminal 7 amino acid sequence to be SDRLNSG- was determined by automatic sequencer and used for synthesizing a degenerated primer. The mRNA from the Gastrodia corm was amplified to be a specific cDNA of about 600 bp through a 3′- RACE experiment with the degenerated primer. The cDNA was directly cloned into pGEM-T vectors and sequenced. The nucleotide sequenceof cDNA and C-terminal amino acid sequence to be -ASG of GAFP-I determined with carboxypeptidase A indicate that the cDNA contains a 429 bp region encoding GAFP-I of 129 aa and a C-terminal extenting peptide of 14 aa. Futhermore, peptides were separated and purified from hydroxylamine cleavaged and α- chymotrypsin digested GAFP-I, and sequenced by automa tic Edman degradation. The determined aa sequence was found to be 98 4% identical to the one deduced from nucleotide sequence of the cDNA. GAFP-I is a single polypeptide of 129 aa, rich in Asn(19)and Gly(14), with a disulfide bridge between the 29th and 52th aa and a calculated Wt of 13958 Da. A high aa sequence similarity was found between GAFP-I and the snowdrop( Galanthus nivalis L.)lectin LECGNA 2, the match of aa was 65%, including an identically located disulfide bridge. The nucleotide sequence of cDNA encoding GAFP-I has been submitted to GenBank database under an accession number AF123309." @default.
- W2384661422 created "2016-06-24" @default.
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- W2384661422 date "1999-01-01" @default.
- W2384661422 modified "2023-09-25" @default.
- W2384661422 title "Primary Structure and cDNA Cloning of the Antifungal Protein GAFP-I from Gastrodia elata" @default.
- W2384661422 hasPublicationYear "1999" @default.
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