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- W2384661659 abstract "To better understand the cleavage efficiency of multiribozyme system on its RNA substrate in the presence and absence of divalent magnesium and monovalent sodium ions, we constructed pGEM-Coat′A,pGEM-Coat′A196Rz plasmids and pGEM-MDR1 target plasmid. They were applied to transcribe RNAs with SP6/T7 transcription kit. Cleavage reactions were carried out in cell-free system and reaction products were analyzed by electrophoresis on 6% denaturing polyacrylamide gels in TBS buffer. The gels were dried and exposed to X-ray films for autoradiography.The Image J software was employed to analyze the dried gels. The results indicated that the cleavage efficiency of the multiribozyme was dependent on the concentration of divalent Mg~ 2+ . The cleavage products increased with the concentrations of divalent Mg~ 2+ and were Mg~ 2+ -concentration and time dependent. No cleavage product was obtained in the presence of lower than 200 mmol/L Na~+alone. On the contrary, monovalent Na~+inhibited the Mg~ 2+ -induced cleavage reaction in Na~+and Mg~ 2+ coexistance. The cleavage rate was significantly lower than that observed with divalent Mg~ 2+ alone. These results suggested that divalent Mg~2+ was required for multiribozyme on substrate cleavage reaction in the physical condition, whereas monovalent Na~+was not." @default.
- W2384661659 created "2016-06-24" @default.
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- W2384661659 date "2008-01-01" @default.
- W2384661659 modified "2023-09-25" @default.
- W2384661659 title "Effects of Divalent Mg~(2+) and Monovalent Na~+ on Cleavage Reactions by Multiribozyme System in vitro" @default.
- W2384661659 hasPublicationYear "2008" @default.
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