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- W2384662030 abstract "Purpose:In order to establish a human tongue cancer cell line resistent to bleomycin(BLM) and to study the relationship between P-gp and MRP expression and drug-resistance. Methods:Human tongue cancer cell line Tca8113 cells were repeatedly treated with high dose of BLM (30(g/ml)to establish the drug-resistant cell line Tca8113/BLM. The expression of P-gp and MRP in Tca8113/BLM and its parental counterpart Tca8113 was analyzed by laser confocus microscope(LCSM) and flow cytometry(FCM). The cell cycle distribution, ADM congregation and effect of reversing agent Progesterone on multidrug resistance(MDR) in both cell lines were detected by FCM. Results:Resistance of Tca8113/BLM cell line to BLM was stable, and its drug-resistance index is 12. It also produced resistance to other chemotherapy drugs. Compared with Tca8113, the doubling time of Tca8113/BLM was prolonged, G_1 phase cell number decreased and S and G_2 phases cell number increased. LCSM observation showed that expression levels of P-gp and MRP in Tca8113/BLM cells were significantly higher than those in Tca8113 cells (P0.01). FCM analysis showed that intracellular concentration of ADM decreased evidently in Tca8113/BLM cells compared with Tca8113 cells, and Progesterone could increase the intracellular concentration of ADM in Tca8113/BLM cells, but progesterone had no significant effect on the intracellular concentration of ADM in Tca8113 cells. Conclusions:A human tongue cancer cell line resistant to BLM was established. Drug-resistance of Tca8113/BLM cells is associated with over-expression of P-gp and MRP. Tca8113/BLM cell line provides an ideal model for MDR research.[" @default.
- W2384662030 created "2016-06-24" @default.
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- W2384662030 date "2004-01-01" @default.
- W2384662030 modified "2023-09-23" @default.
- W2384662030 title "Establishment of human tongue cancer cell line Tca8113/BLM to drug resistance and analysis of mechanism of its drug resistance" @default.
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