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- W2384665005 abstract "The major host protective antigen gene VP 2 of very virulent infectious bursal disease virus(vvIBDV) F9811 was amplified by RT PCR from bursa of one moribund chicken artificially infected vvIBDV F9811,with primers designed according to the published sequence of OKYM in NCBIG.The amplified fragment was about 1.5 kb,and cloned into the SmaⅠ site of pUC119 by blunt ligation.The VP 2 gene of F9811 was sequenced and compared with several vvIBDV strains abroad and domestic.F9811 was demonstrated to be vvIBDV by the fact that its VP 2 gene has 18 nucleotides,but only one amino acid at the 212 th position being different from OKYM.The phylogenetic tree based on the region 143 th~382 th nucleotide revealed that F9811 is a little nearer to OKYM,UK661,HK46 than F9502 and G9303.It implies that many different strains of vvIBDV may exist in China." @default.
- W2384665005 created "2016-06-24" @default.
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- W2384665005 date "2001-01-01" @default.
- W2384665005 modified "2023-09-25" @default.
- W2384665005 title "Cloning and Sequence Analysis of the VP_2 Gene of Very-Virulent Infectious Bursal Disease Virus F9811" @default.
- W2384665005 hasPublicationYear "2001" @default.
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