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- W2384666738 abstract "The cell characters were studied to analyze the reason for difference of the conversion efficiency of Arthrobacter simplex on cortisoniacetas(CA) in the presence of different kinds of organic solvents(ethanol,DMF,DMSO,methanol).The bioconversion rate was determined by the substrate conversion method,the cell survival ratio was calculated by the counting method on plates,the cell metabolic activity was assessed by the glucose metabolic activity retention,AFM was used to observe the cell ultrastructure,FDA method was adopted to determine the cell membrane permeability.Both of the initial conversion rate and the final conversion rate for the biotransformation process were increased by adding four kinds of organic solvents at the concentration level of 4%(volume fraction).The conversion rate for the four kinds of organic solvents decreased in the following order:ethanolDMSOmethanolDMF,the four kinds of organic solvents exerted similar influence upon cell survival ratio and glucose metobolic activity retention,among which DMF was the most influential,DMSO was the secondly,methanol and ethanol was the least.For the integrity of cells,the cell visible change was observed with the addition of methanol and ethanol,and no remarkable change was found with the addition of DMF and DMSO.The cell membrane permeability decreased in the following order:methanolethanolDMSODMF.These results indicated that the difference of conversion rate was resulted from the difference in cell physiological metabolic activity and cell membrane permeability.The increase in cell membrane permeability maybe improve the diffusion of substrate molecules into the cell and their contact with the biological enzyme,which lead to the enhancement of biotransformation process." @default.
- W2384666738 created "2016-06-24" @default.
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- W2384666738 date "2012-01-01" @default.
- W2384666738 modified "2023-09-22" @default.
- W2384666738 title "Study on the Effects of Different Kinds of Organic Solvents on C1,2 Dehydrogenation Reaction by the Analysis of Arthrobacter Simplex Cell Characters" @default.
- W2384666738 hasPublicationYear "2012" @default.
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