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- W2384694634 abstract "An electrochemical DNA sensing system was constructed and investigated with methylene blue(MB) as hybridization indicator.5'-End-SH modified DNA(HS-ssDNA) probes were firstly self-assembled onto the Au-electrode surface,and then hybridized with the complementary single stranded DNA(cssDNA) to form double-stranded DNA system(Au-dsDNA).The results based on differential pulse voltammetry(DPV) showed that cathodic signals of current system were affected by methylene blue(MB) concentration.Furthermore,a thin film formed on Au electrode surface was successfully identified via DPV measurement.Sensitivity of sensing probe was enhanced when the MB concentration ranged from 4.0×10-6 to 1.0×10-5 mol/L,and the optimum concentration of MB was suggested to be 2.0×10-5 mol/L.Recognizability of a single strand DNA with one mismatch indicated a sensing system with high specificity was achieved.The sensitivity was linear and increased when the target DNA concentration ranged from 5.0×10-10 mol/L to 1.8×10-9 mol/L,and the detecting limitation was estimated to be ca 5.0×10-10 mol/L.Moreover,recycling life of the sensing probe was detected to be 5 times." @default.
- W2384694634 created "2016-06-24" @default.
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- W2384694634 date "2008-01-01" @default.
- W2384694634 modified "2023-09-25" @default.
- W2384694634 title "DNA Electrochemical Biosensor with Methylene Blue as Hybridization Indicator" @default.
- W2384694634 hasPublicationYear "2008" @default.
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