FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2384714567> ?p ?o ?g. }

• W2384714567 abstract "Objective To observe the influence of toll-like receptor 4( TLR4) on expression of type 17 helper T cells( Th17 cells) related factors( IL-17A,IL-23 and RORrt) in the liver tissues of rats with ischemia-reperfusion injury,and to investigate the possible mechanisms. Methods Forty healthy male SD rats were divided into 4 groups,10 in each group: the sham operation group,model group,anti-TLR4 group and isotype antibody group. The carotid artery and femoral artery and vein of rats were separated in the sham operation group,and then intubated and injected with the same dose of heparin. Liver damage models of traumatic hemorrhagic shock reperfusion were made in the model group,anti-TLR4 group and isotype antibody group. The anti-TLR4 group and isotype antibody group were respectively injected with antiTLR4 antibody,isotype control antibody 50 μg from femoral vein 10 min before reperfusion,and the pace of injection was slow with microinjector. The rats were executed to obtain the tissues after treatment for 12 h. The TLR4 protein expression in each group was detected by Western blotting; the expression of IL-17A and IL-23 in liver tissues was detected by ELISA; IL-17A,IL-23 and RORrt mRNA changes were observed by RT-PCT. The correlation analysis on IL-17A,IL-23 and RORrt of the model group,anti-TLR4 group and isotype antibody group was conducted. Results The expression of TLR4 protein increased in the model group compared with that of the sham operation group( P 0. 01),and decreased after treatment with anti-TLR4 neutralizing antibodies( P 0. 01),and no significant difference was found in the isotype antibody group as compared with the model group( P 0. 05). Compared with the sham operation group,the expression of IL-17A and IL-23 was increased in the level of protein and mRNA,and RORrt mRNA was increased in the model,antiTLR4 and isotype antibody groups. Compared with the model group,IL-17A and IL-23 expression in the level of protein and mRNA,and the expression of RORrt mRNA was decreased in the anti-TLR4 group( P 0. 05 or P 0. 01). The level of IL-23 was positively correlated with the level of IL-17A,and IL-23 mRNA had a positive correlation with RORrt mRNA. Conclusions Neutralizing TLR4 can decrease the expression of IL-23,IL-17A and RORrt. The mechanism may be that blocking TLR4 can reduce IL-23 expression,and then affect the expression of RORrt which is the specific transcription factor of Th17,thus lead to the reduction of Th17 / IL17 expression in the liver tissues of rats with ischemia-reperfusion injury." @default.
• W2384714567 created "2016-06-24" @default.
• W2384714567 creator A5001583563 @default.
• W2384714567 date "2013-01-01" @default.
• W2384714567 modified "2023-09-28" @default.
• W2384714567 title "Influence of TLR4 on expression of Th17 cells related factors in liver tissues of rats with ischemia- reperfusion injury" @default.
• W2384714567 hasPublicationYear "2013" @default.
• W2384714567 type Work @default.
• W2384714567 sameAs 2384714567 @default.
• W2384714567 citedByCount "0" @default.
• W2384714567 crossrefType "journal-article" @default.
• W2384714567 hasAuthorship W2384714567A5001583563 @default.
• W2384714567 hasConcept C126322002 @default.
• W2384714567 hasConcept C134018914 @default.
• W2384714567 hasConcept C159654299 @default.
• W2384714567 hasConcept C16685009 @default.
• W2384714567 hasConcept C170493617 @default.
• W2384714567 hasConcept C203014093 @default.
• W2384714567 hasConcept C2776070231 @default.
• W2384714567 hasConcept C2777991845 @default.
• W2384714567 hasConcept C2780114680 @default.
• W2384714567 hasConcept C2780678043 @default.
• W2384714567 hasConcept C541997718 @default.
• W2384714567 hasConcept C542903549 @default.
• W2384714567 hasConcept C71924100 @default.
• W2384714567 hasConceptScore W2384714567C126322002 @default.
• W2384714567 hasConceptScore W2384714567C134018914 @default.
• W2384714567 hasConceptScore W2384714567C159654299 @default.
• W2384714567 hasConceptScore W2384714567C16685009 @default.
• W2384714567 hasConceptScore W2384714567C170493617 @default.
• W2384714567 hasConceptScore W2384714567C203014093 @default.
• W2384714567 hasConceptScore W2384714567C2776070231 @default.
• W2384714567 hasConceptScore W2384714567C2777991845 @default.
• W2384714567 hasConceptScore W2384714567C2780114680 @default.
• W2384714567 hasConceptScore W2384714567C2780678043 @default.
• W2384714567 hasConceptScore W2384714567C541997718 @default.
• W2384714567 hasConceptScore W2384714567C542903549 @default.
• W2384714567 hasConceptScore W2384714567C71924100 @default.
• W2384714567 hasLocation W23847145671 @default.
• W2384714567 hasOpenAccess W2384714567 @default.
• W2384714567 hasPrimaryLocation W23847145671 @default.
• W2384714567 hasRelatedWork W2238277951 @default.
• W2384714567 hasRelatedWork W2275593246 @default.
• W2384714567 hasRelatedWork W2316658547 @default.
• W2384714567 hasRelatedWork W2349130974 @default.
• W2384714567 hasRelatedWork W2352027390 @default.
• W2384714567 hasRelatedWork W2363632601 @default.
• W2384714567 hasRelatedWork W2365734886 @default.
• W2384714567 hasRelatedWork W2368685147 @default.
• W2384714567 hasRelatedWork W2369318042 @default.
• W2384714567 hasRelatedWork W2371043085 @default.
• W2384714567 hasRelatedWork W2371130897 @default.
• W2384714567 hasRelatedWork W2375766466 @default.
• W2384714567 hasRelatedWork W2375855583 @default.
• W2384714567 hasRelatedWork W2377055049 @default.
• W2384714567 hasRelatedWork W2393154223 @default.
• W2384714567 hasRelatedWork W2397595173 @default.
• W2384714567 hasRelatedWork W2462534876 @default.
• W2384714567 hasRelatedWork W3031429468 @default.
• W2384714567 hasRelatedWork W3032793414 @default.
• W2384714567 hasRelatedWork W838663234 @default.
• W2384714567 isParatext "false" @default.
• W2384714567 isRetracted "false" @default.
• W2384714567 magId "2384714567" @default.
• W2384714567 workType "article" @default.