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- W2384723792 abstract "Objective (1) To establish SIV p27 hybridoma cell lines.(2) To perform elementary identification of monoclonal antibodies(McAb).Methods BALB/c mice were immunized with recombinant SIV p27 protein.Hybridoma cell lines were set up by using hybridoma technique and semisolid culture medium and monoclonal antibodies were prepared.The hybridoma cell lines were verified by chromosome analysis.The monoclonal antibodies were identified by assessment of cross-reactivity,relative affinity,antigenic epitopes they recognized and the immunoglobulin class determined by Western blot,immunofluorescence assay and enzyme linked immunosorbent assay(ELISA).Results Four hybridoma cell lines that stably secreted SIV p27 McAbs were obtained: 1C3 and 2B6 belonging to IgG1,2E12 belonging to IgG2b,and 3G3 belonging to IgG2a.All four McAbs bound with SIV p27 protein,but had no cross_reactivity with SRV and STLV.However,2B6 and 2E12 had cross_reactivity with HIV p24.The titers of ascites were 1:10240～1:40960, showed by immunofluorescence assay.The averages of chromosome of 1C3,2B6,2E12 and 3G3 were 103,97,96 and 101,respectively.Different antigenic epitopes were recognized by 2E12 and 3G3.Conclusions Four monoclonal antibodies against SIV p27 protein with high specificity and affinity have been successfully generated, which may provide a basis for further establishing immunological assay and for SIV/SAIDS and related performances in AIDS studies." @default.
- W2384723792 created "2016-06-24" @default.
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- W2384723792 date "2006-01-01" @default.
- W2384723792 modified "2023-09-26" @default.
- W2384723792 title "Preparation and Characterization of the Monoclonal Antibody Against SIV p27 Antigen" @default.
- W2384723792 hasPublicationYear "2006" @default.
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