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- W2384753871 abstract "Objective To investigate the influence of an injection of stragalus,a traditional Chinese medicine,on the expression of TNF-α when IgG anti-endothelial cell antibodies(AECA) that had been cultured in a serum obtained from diabetic retinopathy(DR) patients were stimulated by endothelial cells cultivated in vitro.Methods Three groups were established based on different concentrations of antibodies and two groups on different concentrations of medication.These groups were then stimulated by human umbilical vein endothelial cells(HUVEC) cultured in vitro and purified IgG AECA cultured in the serum of DR patients using ion exchange chromatography.Stragalus was injected into it.Then the toxic effect of TNF-α on the three groups was measured when IgG AECA stimulated the HUVEC cells using 3-(4,5-dimethy thioazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) established by WEHI 164.13 cells which were highly sensitive to TNF-α.Results The quantity of TNF-α in the experimental groups of astragalus was remarkable lower than that in the control group(P0.05).Moreover,those in the groups with high concentrations were significantly higher than those in the groups of low concentrations(P0.05).Conclusion Astragalus had significant inhibitory effects on the expression of TNF-α produced when IgG AECA in serum from diabetic retinopathy patients was stimulated by endothelial cells cultured in vitro." @default.
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- W2384753871 date "2006-01-01" @default.
- W2384753871 modified "2023-09-25" @default.
- W2384753871 title "An investigation of the inhibitory effects of stragalus injection on the TNF-α expressed by endothelial cells" @default.
- W2384753871 hasPublicationYear "2006" @default.
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