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- W2384756790 abstract "【Objective】 This research aimed to find disease-susceptible and resistant protein by proteomics comparison of susceptible and resistant grape leaves.【Method】 With the material of disease resistant plant 6-12-4 and susceptible plant 6-12-2,their leaves were artificially inoculated by grape powdery mildew and then picked 0,2 and 4 days after inoculation.The total proteim were extracted from the sample leaves and their proteomic differences were analysed by two dimension electrophoresis.Differntially expressed protein dots were recycled,purified and identified by MALDI-TOF Mass method.【Result】 Totally 26 differentially expressed protein spots were obtained,10 of the most differentially expressed proteins were identified by MS.7 out of 10 were positive,and the rest negative.Peptide fragment sequencing result showed that among these 7 positive proteins,two positive spots were hypothetical proteins,and the other two unnamed proteins,spot 5411 was oxygen evolving complex 33 ku photosystem II protein,spot 5625 was ribulose-1,5-bis-phosphate carboxylase/oxygenase activase 1,Rubisco;spot 7121 was pathogensis related protein,PR10.【Conclusion】 These proteins were somehow associated with the disease-resistant and disease-susceptible characteristics of grape." @default.
- W2384756790 created "2016-06-24" @default.
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- W2384756790 date "2012-01-01" @default.
- W2384756790 modified "2023-09-23" @default.
- W2384756790 title "Proteomics difference of powdery mildew resistant and susceptible grape leaves" @default.
- W2384756790 hasPublicationYear "2012" @default.
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