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- W2384769123 abstract "Objective To clone the recombinant plasmid of small interfering RNA(siRNA) against Fas gene and to evaluate its activity of inhibiting the Fas expression in human lung carcinoma A549 cells,and explore the mechanism of Fas/FasL pathway on lung cancer metastasis.Methods Three Fas siRNA template DNA sequences were designed and synthesized.The annealed siRNA template was inserted into pGCsi-U6 plasmid.which was transfected into A549 cells with limpofectin 2000,The effect of pSi-Fas on the Fas expression of human lung carcinoma A549 cells was detected by Western blot.Results It was confirmed by restrictive enzyme digestion and sequence analysis that the recombinant plasmid was cloned and the aim sequence was obtained.The Fas expression was inhibited at protein levels by the recombinant pSi-Fas.Conclusion Fas siRNA expression plasmid pSi-Fas successfully constructed,which can inhibit the expression of Fas protein in human lung carcinoma A549 cells." @default.
- W2384769123 created "2016-06-24" @default.
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- W2384769123 date "2008-01-01" @default.
- W2384769123 modified "2023-09-24" @default.
- W2384769123 title "Construction of Fas siRNA expression plasmid and its ability to inhibit Fas expression" @default.
- W2384769123 hasPublicationYear "2008" @default.
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