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- W2384785004 abstract "OBJECTIVE To observe the effect of Adjusted Dahuang Zhechong Pill on proliferation,migration,and expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 2 (MMP-2) of human retinal pigment epithelial cell line (CRL-2302) cells in vitro in order to understand the action pathway and mechanism of Adjusted Dahuang Zhechong Pill interfering on choroidal noevascularization (CNV). METHODS MTS method was used to access the effect of Adjusted Dahuang Zhechong Pill on the proliferation of CRL-2302 cells induced by VEGF. Transwell insert was used to access the effect of Adjusted Dahuang Zhechong Pill on the migration of CRL-2302 cells. Western Blot and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to detect Adjusted Dahuang-Zhe-chong Pill on the expression of VEGF and matrix metalloproteinase 2 (MMP-2) protein and mRNA of CRL-2302 cells. RESULTS The proliferation of CRL-2302 cells induced by VEGF was inhibited by Adjusted Dahuang Zhechong Pill at concentrations of 0.05,0.1,and 0.2 mg/ml. When the concentration of Adjusted Dahuang-Zhechong Pill was 0,12.5,25,and 50 mg/ml,cells number that migrated to under the Transwell membrane was 198.33±19.86,121.67±14.05,62.33±11.24,and 18.67±4.51 respectively. At con-centrations of 50 and 100 mg/ml,Adjusted Dahuang Zhechong Pill could inhibit the expression of VEGF and MMP-2 protein and mRNA of CRL-2302 cells. At concentrations of 200 mg/ml,Ad-justed Dahuang Zhechong Pill could inhibit the protein expression of MMP-2 and VEGF and mRNA expression of MMP -2 of CRL-2302 cells. CONLUSIONS Adjusted Dahuang Zhechong Pill could inhibit CNV concerned with CRL-2302 cells through the path of suppression of proliferation,migration of CRL-2302 cells. The inhibition of the expression of VEGF and MMP-2 protein and mRNA of CRL-2302 cells may be one of the important mechanisms." @default.
- W2384785004 created "2016-06-24" @default.
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- W2384785004 date "2009-01-01" @default.
- W2384785004 modified "2023-09-26" @default.
- W2384785004 title "An experimental study on the proliferation and migration of retinal pigment epithelial cells inhibited by Adjusted Dahuang Zhechong Pill and its mechanism" @default.
- W2384785004 hasPublicationYear "2009" @default.
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