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- W2384785110 abstract "【Objective】The objective of this study is to develop broad specificity polycolonal antibodies against a group of organophosphorous pesticides.【Method】 Diethylphosphono acetic acid (DPA) was used as a generic hapten, and conjugated to bovine serum albumin (BSA) by the mixed-anhydried method and EDC method. Six New Zealand white rabbits were immunized for preparation of antiserum.【Result】The titers of antiserum by prepared the two methods had reached 25 600 and 6 400, respectively. The CI-ELISA was conducted with the antiserum, the detection limit for DPA was 0.0035 μg·ml-1 and the I50 was 0.182 μg·ml-1. The antiserum also showed high affinities to chlorpyrifos, omethoate, diazinon, parathion-ethyl, profenofos, and phoxin, the I50 to these pesticides were 0.12, 0.21, 0.24, 0.78, 0.97, 3.8 μg·ml-1. 【Conclusion】It is concluded that this assay is a suitable screening tool for quantities and semi-quantities detection of the five organophosphorous pesticides." @default.
- W2384785110 created "2016-06-24" @default.
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- W2384785110 date "2008-01-01" @default.
- W2384785110 modified "2023-09-23" @default.
- W2384785110 title "Use of Generic Hapten in Production of Broad Specificity Anti-Organophosphorous Pesticides Antibody" @default.
- W2384785110 hasPublicationYear "2008" @default.
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