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- W2384799060 abstract "Oleate desaturase is the rate limiting enzyme of the desaturation of linoleic acid in the production of an essential fatty acid. The 1.14 kb fragment in plasmid pRSET/HO-A encoding oleate desaturase was subcloned into the yeast-Escherichia coli shuttle vector pYES6, thus an expression recombinant plasmid pYES/HO-A contatining target gene was constructed. The pYES/HO-A was introduced into defective mutant INCSc1 of Saccharomyces cerevisiae by LiAc method. Oleate desaturase activity was expressed under appropriate media and temperature conditions, the level of linolenic acid reached 18.6% of the total yeast fatty acids by GC-MS detection, and the function of oleate desaturase in S. cerevisiae was verified. Total fatty acids was extracted and esterified, then analyzed by gas chromatography. A novel peak corresponding to linoleic methyl ester standards was detected with the same retention time. GC-MS analysis demonstrsted that the novel peak was linoleic acid methyl ester. These results exhibited Δ12 fatty acid desaturase activity, converting oleicto linoleic specifically." @default.
- W2384799060 created "2016-06-24" @default.
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- W2384799060 date "2007-01-01" @default.
- W2384799060 modified "2023-09-26" @default.
- W2384799060 title "Function Expression of Oleate Desaturase Gene from Peanut in Sacchromyces cerevisiae" @default.
- W2384799060 hasPublicationYear "2007" @default.
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