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• W2384804970 abstract "A bacterial strain GXN151 which can efficiently degrade natural cellulose such as rice straw and bagasse, was isolated from rice straw haystack at the farmland of Guangxi University The strain was identified as Bacillus licheniformis by morphology, 16S rRNA gene sequence analysis and testing for utilization of propionate A genomic library of GXN151 was constructed in Escherichia coli JM109 by ligating BamHI-digested pBluescript KS(+) vector with recovered 3~10 kb Sau3AI-partially digested GXN151 DNA The library contains 7 099 clones with biggest insert of 110 kb, smallest insert of 31 kb, and the average insert size of 46 kb The library has 996% chances (P=996%)to cover any given gene in the genome of GXN151 Eight clones expressing carboxymethylcellulase(CMCase) activity were isolated by screening this library DNA sequencing and PCR-based screening of the eight clones revealed that these clones fell into 3 types of non-overlapping independent clones Sequencing analysis revealed that cel5A gene on pGXNL2 was 1 626 bp, encoding an enzyme with 542 amino acids The N-terminal 42 amino acids of Cel5A was characteristic of signal peptide Amino acid 66～321 of Cel5A formed family 5 glycosyl hydrolase catalytic domain, and amino acid 391～472 of the enzyme formed family 3 carbohydrate-binding module(CBM) This is the first report that Bacillus licheniformis can degrade natural cellulose and a cellulase gene was cloned from this bacterium" @default.
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• W2384804970 date "2003-01-01" @default.
• W2384804970 modified "2023-09-25" @default.
• W2384804970 title "Isolation and identification of a natural cellulose degrading Bacillus licheniformis strain GXN151 and cloning and sequencing of a cellulase gene cel5A from the bacterium" @default.
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