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- W2384828663 abstract "The specific fragment of Pneumococcal surface protein A(PspA)and Pneumococcal Surface Adhesin A(PsaA)gene was amplified by PCR from Streptococcus pneumonia 5 and Streptococcus pneumonia 19.The amplified fragnent of PspA and PsaA gene was ligated into pET-27b(+)vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA.Induced by IPTG,the expression level was as high as 75 % of the total disolube protein.The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E.coli BL 21,which showed the good immunogenicity and a broadly cross reactivity with the other serotypes." @default.
- W2384828663 created "2016-06-24" @default.
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- W2384828663 date "2008-01-01" @default.
- W2384828663 modified "2023-09-23" @default.
- W2384828663 title "Study of Expression of Pneumococcal Surface Protein and Immunogenicity" @default.
- W2384828663 hasPublicationYear "2008" @default.
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