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- W2384841279 abstract "Objective To establish a novel enzyme-linked immunosorbent assay(ELISA) for quantitative detection of the concentration of anti-thyroid peroxidase antibody(TPOAb) in serum.Methods The microtiter plate was coated with biotinylated bovine serum albumin(BSA) and streptavidin.The biotinylated TPO antigen and standardized anti-TPOAb or test sera were successively added into the wells of plate.The HRP-anti-IgG was then added into the plate for colorization.The optimal concentrations of biotinylated TPO antigen and HRP-anti-IgG were screened by chessboard titration,and the reaction conditions were optimized for the method evaluation.Results In the indirect-coating mode,the amount of coated antigen was 0.083 μg/mL.The sensitivity of the assay was 0.165 IU/mL.The coefficient of variations(CV) of inter-assay in high and low concentration of serum mixture were 9.2% and 9.0%,and the CV of intra-assay were 4.6% and 5.6% respectively.The recovery rate was between 96% and 104%.The coated ELISA plate remained stable for 5 days at 37 ℃.The rate of cross-reaction with anti-thyroid globulin(TGAb) was 0.22%.The reference range in serum was less than 65.7 IU/mL.The correlation coefficient of the experimental results with those of Abbott kit was 0.985(P0.01).Conclusion In the developed ELISA of indirect-coated mode,the amount of needed purified antigen significantly reduced.The sensitivity and specificity of the assay were satisfied.The method is simple and cost-saving,so it should be very suitable for anti-TPOAb detection in primary hospitals." @default.
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- W2384841279 date "2011-01-01" @default.
- W2384841279 modified "2023-09-26" @default.
- W2384841279 title "A novel enzyme-linked immunosorbent assay for quantitative detection of anti-thyroid peroxidase antibodies in serum" @default.
- W2384841279 hasPublicationYear "2011" @default.
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