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- W2384860120 abstract "Objective: To discuss the effects of MTDH gene on the expression of angiogenesis maker Hypoxia-inducible factor-1α and Vascular Endothelial Growth Factorin in the breast cancer. Methods: UsingRNA interference plasmid that can express shRNA targeting MTDH to transfect MCF-7 breast cancer cells. The result of knockdown MTDH gene was confirmed by RT-PCR and Western blot. The expression of HIF-1α and VEGF protein before and after transfection was determined by Western blot. The proliferation of MCF-7 cells after downregulation of MTDH was detected by MTT. Results: The transfection efficiencies of plasmids were measured using fluorescence microscope at 48 hours after transfection in MCF-7 cells. The result showed that transfection rate of plasmids was70%. The mRNA and protein level of MTDH were abate in MTDH-shRNA group, and the level ofHIF-1α and VEGF protein was decreased as compared with controls, with statistical significance(P0.05). The proliferation of MCF-7 cells was suppressed, with statistical significance(P0.05). Conclusion: Downregulation of MTDH inhibits expression of HIF-1α and VEGF in MCF-7 breast cancer cells,and suppresses cell proliferation, prompting that MTDH maybepromoteangiogenesis in human breast cancer." @default.
- W2384860120 created "2016-06-24" @default.
- W2384860120 creator A5025937753 @default.
- W2384860120 date "2013-01-01" @default.
- W2384860120 modified "2023-09-28" @default.
- W2384860120 title "Downregulation of MTDH Using short Hairpin RNA Inhibits the Expression of HIF-1α and VEGF in MCF-7human Breast Cancer Cells" @default.
- W2384860120 hasPublicationYear "2013" @default.
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