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- W2384879906 abstract "Objective:To study the gene expression of vitamin k dependent carboxylase (VKDC) in patients with calcium oxalate urolithiasis and evaluate the correlation between the quantity of VKDC and the formation of calcium oxalate calculus.Methods:Using Taqman real-time reverse transcription-polymerase chain reaction (RT-PCR) and 2-△△Ct method, the expression of VKDC mRNA were evaluated in renal tissue specimens from patients with calcium oxalate urolithiasis (urolithiasis group, n=19) and non-urolithiasis (control group, n=21). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the housekeeping gene.Results:The amount of VKDC mRNA in urolithiasis group was lower than in the controls, indicated by significantly higher delta threshold cycles (△Ct) in RT-PCR for the VKDC gene in the urolithiasis renal tissue, with mean ± SD of 8.996±1.667 and 6.023±1.234(P0.01), respectively. VKDC mRNA was on average 7.86-fold(range, 2.47 to 24.93-fold)underexpressed in urolithiasis group compared to controls. Conclusions:These data demonstrate that the gene expression of VKDC is decreased in renal tissues of patient with calcium oxalate urolithiasis, and may be involved in the formation of calcium oxalate calculus." @default.
- W2384879906 created "2016-06-24" @default.
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- W2384879906 date "2007-01-01" @default.
- W2384879906 modified "2023-09-28" @default.
- W2384879906 title "Gene expression of vitamin k dependent carboxylase in patients with calcium oxalate urolithiasis" @default.
- W2384879906 hasPublicationYear "2007" @default.
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