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- W2384927072 abstract "Objective To explore the applicable value of automated capillary zone electrophoresis in hemoglobin analysis.Methods By using French Sebia Capillarys 2 electrophoresis system(Version 5.50) and related reagents,the whole blood samples were treated by the system automatically, and under the condition of 9.8 kV,34℃,pH9.4 of buffer solution,hemoglobin electrophoresis was carried out within eight parallel capillaries(17.5 cm×25μm) and the percentage concentration of each component were directly detected at 415 nm.Results By analyzing the results of hemoglobin electrophoresis of 183 healthy adults,HbA_2 reference range of our laboratory was established:2.1%-3.2%. Precision test was performed by quality control of Hb AFSC,and CVs about each component ranged from(intra-assay:0.21%to 1.07%;inter-assay:0.60%to 3.10%).In 6045 samples which needed diagnosis and differential diagnosis of hemolytic jaundice clinically,379 cases were detected with higher HbA2,203 cases were detected with higher HbF,27 cases were with HbH band,8 cases with HbE band,4 cases with HbS band,3 cases with Hb J-K band,2 cases with Hb Bart's band,and 1 case was with HbD band.Conclusion Sebia Capillarys 2 electrophoresis system can clearly distinguish higher HbA_2,higher HbF and other abnormal bands;can accurately distinguish HbC from HbE and HbA_2;can also perfectly distinguish and focalize on HbF between HbA and HbS;and can easily distinguish HbS and HbD which have the close migration speed.With a high degree of automation,easy operation,rapid and accurate characteristics,it seems appropriate for conventional application of hemoglobin analysis in clinical laboratories of medical institutions." @default.
- W2384927072 created "2016-06-24" @default.
- W2384927072 creator A5061828492 @default.
- W2384927072 date "2009-01-01" @default.
- W2384927072 modified "2023-09-23" @default.
- W2384927072 title "Clinical application of fully automated multicapillary zone electrophoresis in hemoglobin analysis" @default.
- W2384927072 hasPublicationYear "2009" @default.
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