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- W2384927503 abstract "It is the very significant and useful to distinguish hydrogen-producing bacteria and guide the start-up, operation of hydrogen-producing reactor by end liquid products of gas chromatography analysis. The 3 strains of hydrogen-producing bacteria were isolated from the activated sludge by improved Hugate' rolling tube technique and plate method of culture bottle and HPB-LR medium which was special for isolating fermentative hydrogen-producing bacterium in our lab. The 3 strains were named as R3,RL20,RL37. Another was no hydrogen-producing bacterium and the name was RL16. The mixed bacteria was compared with the pure strains. The fermentative end liquid products of the pure cultures and mixed bacteria are analysed by gas chromatography. It was found that there were ethanol, acetic acid, propionic acid, butanic acid, phocenic and lactic acid. The proportion of ethanol and acetic acid of hydrogen-producing bacteria was 99. 0%～100. 0% of total end products, which belonged to classical ethanol-type fermentation. The total content of ethanol and acetic acid was equal to that of propionic acid in the acidic end products of the no hydrogen-producing bacteria, which belonged to propionic acid type fermentation. Though there were some differences on the characteristics of physiologic metabolism between pure hydrogen-producing bacteria and mixed bacteria, the main type of metabolism wasn't changed and still was ethanol-type fermentation. It was concluded that the GC analysis of liquid acidic end products may preliminarily determined the fermentative type of bacteria, which were isolated from hydrogen bio-production reactor. If the proportion of ethanol and acetic acid of bacteria were 95%～99% in the total acidic end products, it may determine that the bacteria were fermentative hydrogen-producing bacteria." @default.
- W2384927503 created "2016-06-24" @default.
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- W2384927503 date "2005-01-01" @default.
- W2384927503 modified "2023-09-26" @default.
- W2384927503 title "Study on judging hydrogen-producing bacteria by gas chromatography analysis of acidic end products" @default.
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