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- W2384967710 abstract "Objective:To explore proteomic changes in osteoblasts induced by calcitonin gene-related peptide(CGRP).Methods:Cultured MG63 osteoblasts in exponential phase of growth were randomly divided into two groups:Cells in CGRP group were treated with CGRP at 10-7~10-10 mol/L.Those in the control were cultured without CGRP.After protein extraction,2-D electrophoresis(2-DE)was employed to separate proteins,and 6 protein spots expressed differentially were picked out and subjected to tryptic in-gel digest and matrix——assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF)for identification.Results:967±17 proteins were detected and the match rate was(85.1±1.4)%.6 protein points displayed significant changes in CGRP treated osteoblasts,2 decreased in expression and 4 disappeared.5 proteins identified after mass spectrometry including p180/ribosome receptor associated with ribosome fixing and new protein transposition,HRNR Hornerin associated with Ca2+ accommodation,and so on.Conclusion:The proteome in osteoblasts induced by CGRP alters dramatically,decreased and disappeared protiens are related to the changes of Ca2+ accommodation,ribosome fixing and new protein transposition in osteoblasts." @default.
- W2384967710 created "2016-06-24" @default.
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- W2384967710 date "2010-01-01" @default.
- W2384967710 modified "2023-09-26" @default.
- W2384967710 title "Proteomic differential expression of osteoblasts induced by CGRP" @default.
- W2384967710 hasPublicationYear "2010" @default.
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