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- W2384991237 abstract "Objective To investigate the effects of polyethylene glycol 400 on the biological characteristics of corneal epithelial cells(CECs) and molecular mechanisms thereof.Methods Polyethylene glycol 400 was diluted to 0.1% with DMEM/F12 medium.CECs were cultured in this medium and cell morphology was recorded using an inverted optical microscope after 24,48 and 72 hours.Cell growth curves were measured by using MTT assay and cell cycle was determined by using flow cytometer;RT-PCR was used to measure the expression of basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF) in CECs.Results 0.1% polyethylene glycol 400 had no discernable effects in the morphology of CECs.CECs cultured in the dilution of polyethylene glycol 400 solutions have the same polygon cell morphology as the control group.Results from MTT assay and cell cycle measurements showed that 0.1%polyethylene glycol 400 promotes in vitro HCEC growth(P0.01)and up-regulates the expression of bFGF and EGF in cultured CECs(P0.05 or 0.01).Conclusion 0.1% polyethylene glycol 400 does not affect cell morphology and it promotes CEC proliferation via reducing cell cycle and increasing bFGF and EGF expression." @default.
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- W2384991237 date "2012-01-01" @default.
- W2384991237 modified "2023-09-23" @default.
- W2384991237 title "The effects of polyethylene glycol 400 on corneal epithelial cell growth in vitro" @default.
- W2384991237 hasPublicationYear "2012" @default.
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