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- W2384996609 abstract "Objective Peroxisome proliferator-activated receptor(PPAR-γ)has a comprehensive anti-cancer activity,whether PPAR-γ anti-tumor effect is mediated by nitric oxide(NO)pathway is not clear at present.This study aimed to investigate the influence and significance of PPAR-γ on NO production in Jurkat human T lymphoma cell line.Methods Jurkat cells were stimulated with 20 μmol /L of rosiglitazone(PPAR-γ activator)in experimental group.Rosiglitazone was not added in the cells in control group.NO content was detected by using nitrate reductase and PPAR-γ mRNA expression was detected by RT-PCR 6 h,12 h,18 h,24 h and 30 h after treatment.Results The expressions of PPAR-γ mRNA were increased gradually with time after medication.NO contents were significantly higher than those in the control group 6 h,12 h,18 h,24 h and 30 h after treatment and the expressions of PPAR-γ and the contents of NO showed positive correlation(r=0.87,P0.05).Conclusion PPAR-γ activator stimulates NO production in a time-dependent mode in Jurkat cells.It is suggested that PPAR-γ might adjust target factors by NO pathway in Jurkat cells." @default.
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- W2384996609 date "2009-01-01" @default.
- W2384996609 modified "2023-09-23" @default.
- W2384996609 title "Study on the time effect of NO production induced by PPAR-γ ligand in Jurkat cells" @default.
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