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- W2384996933 abstract "OBJECTIVE To prepare a soluble immobilized papain and determine its enzymatic properties.METHODS The soluble immobilized papain was obtained by conjugating the papain with the hydrophilic high molecular polymer carboxylmethyl cellulose acetic succinate.Then the optimum pH and temperature,the Km value,the thermodynamic stability,the acid-base stability、the low temperature stability and operational stability of immobilized and soluble papains were determined,respectively.RESULTS The optimun pH of soluble and immobilized papain were 6.0 and 5.0.Their optimum temperatures were 60℃ and 70℃.Their Km values were 2.53 and 3.07 mg·ml-1.Under 60℃,after 12 hours the relative activity of immobilized papain was 62%.Under 4℃,after 30 days the relative activity was still over 90%.Under the pH6.0,after 72 hours the activity of immobilized papain didn't decrease,and betweeen pH4 and 7 its stability was high(relative activity was over 80%).CONCLUSION Soluble immobilized papain can fully solubilize above the pH5.5.After immobilization the optimum pH range of papain decreased,and its optimum temperature,Km value and stabilities were all higher than before.Its thermodynamtic stability and the acid-base stability were also strengthened than before." @default.
- W2384996933 created "2016-06-24" @default.
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- W2384996933 date "2007-01-01" @default.
- W2384996933 modified "2023-09-28" @default.
- W2384996933 title "Preparation and properties of soluble immobilized papain" @default.
- W2384996933 hasPublicationYear "2007" @default.
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