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- W2385029557 abstract "AIM:To isolate,culture and purify the rats' apical bud cells and observe the expression pattern of Notch 1 in the apical bud cells cultured in vitro.METHODS:We separated the lower incisor germs from postnatal 7 d SD rats,cut the labial apical part of these germs and cultured the mixed cells by enzymatic digestion.Then,the apical bud cells were purified by differential trypsinization and identified by immuno-staining of cytokeratin 14 and vimentin.The Notch 1 expression was examined by immunocytochemistry.RESULTS:The apical bud cells were purified after 2-3 times of differential trypsinization,and were positive stained for cytokeratin 14 and negative for vimentin.CONCLUSION:Notch 1 is positively expressed by some special apical bud cells cultured in vitro,which indicates that Notch pathway may be involved in the maintenance of the apical bud cells growth." @default.
- W2385029557 created "2016-06-24" @default.
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- W2385029557 date "2008-01-01" @default.
- W2385029557 modified "2023-09-27" @default.
- W2385029557 title "Notch 1 expression in the rats' apical bud cells cultured in vitro" @default.
- W2385029557 hasPublicationYear "2008" @default.
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