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- W2385030833 abstract "The complementary DNA of 1.7 kb HA gene was prepared from the cDNA by RT-PCR.The amplified fragment was cloned into the plasmid vector pMD18-T and then sequenced.The sequence coding of signal peptide HA was deleted,then subcloned into the downstream of the baculovirus transfer vector pBlueBacHis that contain the honeybee melittin secretion signal.The recombinant transfer vector was screened at random by picking colonies and designated pBHisH7HA.The pBHisH7HA was sequenced and co-transfected the sf9 cell with the Bac-N-BlueTM DNA by the technique of cationic liposome mediated transfection.The recombinant baculovirus was purified by three cycles of plaques assay with the chromogenic substracte X-gal in the low temperature melting agarose,and identified by PCR?HA test?SDS-PAGE and Western blot test,which proved it have good biological activity." @default.
- W2385030833 created "2016-06-24" @default.
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- W2385030833 date "2006-01-01" @default.
- W2385030833 modified "2023-09-23" @default.
- W2385030833 title "Expression of avian influenza virus A/PFV/Restock/34(H7N1) HA gene in recombinant baculovirus" @default.
- W2385030833 hasPublicationYear "2006" @default.
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