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- W2385036430 abstract "Objective: The fruit of Gardenia jasminoides produce and accumulate of crocin,which is derived from zeaxanthin.We investigated the molecular mechanism involved in the synthesis of crocin by cloning the phytoene synthase(PSY) in fruit of G.jasminoides,the PSY is the first committed step in carotenogenesis.Method: The full-length cDNA encoding putative phytoene synthase(GjPSY) was cloned from G.jasminoides fruit cDNA library.The cDNA encoding GjPSY was cloned into the expression vector pET-21b.The recombinant protein was expressed in E.coli BL21(DE3) cells and subsequently purified using Ni2 +-affinity chromatography.Result: The GjPSY cDNA is 1876 bp,contains a predicted 1314 bp open reading frame that encodes 437 amino acids.Specific band of the purified fusion protein appeared at about 50.1kDa was detected by SDS-PAGE.Conclusion: GjPSY protein share 55% to 93% amino acid identity with phytoene synthases from other plants.The 3D model structure of GjPSY revealed typical PSYs tertiary structures.The recombinant vector can be used for GjPSY function assays." @default.
- W2385036430 created "2016-06-24" @default.
- W2385036430 creator A5041746901 @default.
- W2385036430 date "2013-01-01" @default.
- W2385036430 modified "2023-09-27" @default.
- W2385036430 title "Molecular Cloning and Prokaryotic Expression of GjPSY Gene from Gardenia jasminoides" @default.
- W2385036430 hasPublicationYear "2013" @default.
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