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- W2385047715 abstract "Objective To constructe recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1(EOLA1),which would be used in the following research for promoter determination.Methods Genomic DNA of human endothelial cell was prepared,and specific primers were designed to clone different length of the upstream regions.These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.These recombinant reporter gene were digested with restriction enzyme and determined the sequence.Results Three expression plasmid of pGL3-EOLA1-Luc fluorescein with different length of upstream gene were constructed successfully,and the recombinant of promoter of EOLA1 were obtained.Conclusion The reporter gene system of EOLA1 upstream gene with different length offer the substructure for analysing the activity of EOLA1 promoter for further research." @default.
- W2385047715 created "2016-06-24" @default.
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- W2385047715 date "2008-01-01" @default.
- W2385047715 modified "2023-09-23" @default.
- W2385047715 title "Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1" @default.
- W2385047715 hasPublicationYear "2008" @default.
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