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- W2385066840 abstract "To clone the M gene of SARS Coronavirus,express in E.coli and purify the expression products,the M gene fragment of SARS Coronavirus was amplified and cloned into the pMD-18T vector.After nucleotide sequencing,the M gene fragment was subcolned into the expression vector pET23a with correct orientation and then transformed into E.coli DH5α.The plasmid from the corrected clone identified by PCR and endonuclease digestion was transformed into E.coli BL21(DE3)and then induced for expression.The expression product was purified by metal-chelated affinity chromatography and analyzed by Western blotting.It was found that the 675bp gene fragment was amplified by RT-PCR.Nucleotide sequencing showed there was no sense mutation.A 27KD protein was expressed after induction and purified by metal-chelated affinity Chromatography.Western blotting result indicated that the expressed products could be recognized by sera of SARS Patinats.M gene of SARS Coronavirus was successively cloned and expressed in E.coli and the expressed gene products were successfully purified and recognized by SARS patient's is serum." @default.
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- W2385066840 date "2005-01-01" @default.
- W2385066840 modified "2023-09-25" @default.
- W2385066840 title "Cloning,expression and purification of the M gene from SARS Coronavirus" @default.
- W2385066840 hasPublicationYear "2005" @default.
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