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- W2385087127 abstract "Objective:To develop an HPLC method for determination of contents of five flavonoids(narirutin,hesperidin,nobiletin,3,5,6,7,8,3',4'-heptanmethphoxyflavone,tangeretin)in Pericarpium Citri Reticulatae,and to accomplish the cluster analysis of contents.Method:HPLC was performed on a Waters Symmetry C18 analytical column(250 mm×4.6 mm,5 μm)with gradient elution of 0.1% aqueous formic acid and acetonitrile at the rate of 1.0 mL·min-1.The diode array detection was set at 300 nm.Results:Narirutin,hesperidin,nobiletin,3,5,6,7,8,3',4'-heptanmethphoxyflavone and tangeretin had good linearity in the ranges of 1.00-9.00 μg·mL-1(r=0.9998),1.00-9.00 μg·mL-1(r=0.9999),0.10-0.90 μg·mL-1(r=0.9997),0.10-0.90 μg·mL-1(r=0.9999)and 0.10-0.90 μg·mL-1(r=0.9994),respectively;The average recoveries(n=5)were 96.8%,99.4%,93.3%,94.4% and 95.8%,respectively.Conclusion:The developed method is rapid,accurate with high repeatability.The results showed that the significant difference in samples from different provinces had been observed.The results also showed multiple chemical markers provided more comprehensive information than unique marker for quantitative analyses of Pericarpium Citri Reticulatae." @default.
- W2385087127 created "2016-06-24" @default.
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- W2385087127 date "2009-01-01" @default.
- W2385087127 modified "2023-09-26" @default.
- W2385087127 title "HPLC simultaneous determination of contents of five flavonoids in Pericarpium Citri Reticulatae" @default.
- W2385087127 hasPublicationYear "2009" @default.
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