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- W2385087255 abstract "Objective To investigate the mutations of the K76T position of Pfcrt gene of Plasmodium falciparum in the imported cases reported in Hefei.Methods Blood samples were collected from 11 patients infected with Plasmodium falciparum.A nested PCR(polymerase chain reaction) method was applied to amplify the Pfcrt gene and template DNA from Plasmodium falciparum in blood spot specimens.The amplified PCR products were evaluated after digestion by restriction enzyme.Results Six blood samples had been amplified successfully in the pool.Four of the six blood samples carrying the mutant allele at codon K76T were not digested,while the remaining 2 blood samples with wild allele K76T were digested.Conclusion The imported Plasmodium falciparum cases in Hefei have a high mutation rate of K76T position.Therefore,drug resistance in the imported Plasmodium falciparum treatment should deserve more attention in the future." @default.
- W2385087255 created "2016-06-24" @default.
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- W2385087255 date "2013-01-01" @default.
- W2385087255 modified "2023-09-23" @default.
- W2385087255 title "Mutations of K76T positions of Pfcrt Gene in the Imported Plasmodium falciparum cases in Hefei" @default.
- W2385087255 hasPublicationYear "2013" @default.
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