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- W2385122156 abstract "【Objective】To construct a lentiviral vector of RNA silence interference (siRNA) of TIEG.【Methods】 The effective sequence of siRNA targeting TIEG was confirmed in our previous study. The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed synthesized and cloned into the PshRNAcopGFP -lentivector which contained H1 promoter and green fluorescent protein (GFP). The resulting lentiviral vector containing TIEG shRNA was named LV-psiRNA-TIEG, and it was confirmed by PCR and sequencing 293T cells were cotransfected with psiRNA-TIEG and lentivirus packaging plasmids mixtures. All virus stocks were produced by transfection Reagent Lipofectmaine 2000. The titer of virus was tested according to the expression level of GFP. The rats were injected through tail vein with virus solution, we detected expression levels of TIEGmRNA of rat renal tissue by RealTime-PCR. 【Results】PCR and DNA sequencing demonstrated that the lentivirus RNAi vector of TIEG producing psiRNATIEG was constructed successfully. The titer of concentrated virus was 1× 105 ifu/μL. TIEGmRNA expression levels of the experimental rats were significantly lower than the control group (50.7%).【Conclusions】TIEGsiRNA lentiviral vector psiRNA-TIEG was constructed successfully." @default.
- W2385122156 created "2016-06-24" @default.
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- W2385122156 date "2009-01-01" @default.
- W2385122156 modified "2023-09-26" @default.
- W2385122156 title "Construction and identification of lentiviral vector of RNA interference of TIEG gene" @default.
- W2385122156 hasPublicationYear "2009" @default.
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