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- W2385148624 abstract "Objective To investigate the possible mechanism of the fibroblasts inducing the vascularization of dermal substitute. Methods Fibroblasts were seeded on the surface of acellular dermal matrix and cultivated in vitro to construct the living dermal substitute. The release of interleukin 8 (IL 8) and transfonming growth factor β 1(TGF β 1) in culture supernatants were assayed by enzyme linked immunosorbent assay, the mRNA expression of acid fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) were detected by RT PCR. Then, the living substitute was sutured to full thickness excised wound on BALB/C mice, and the fate of fibroblast was observed by using in situ hybridization. Results Fibroblasts cultured on acellular dermal matrix proliferated and reached a single layer confluence. Fibroblasts could secret IL 8(192.3±15.9)pg/ml and TGF β 1(1.105±0.051)pg/ml. There was the mRNA exparession of aFGF and bFGF. Fibroblasts still survived and proliferated 3 weeks after grafting. Conclusion Peptides secreted by fibroblasts and its survival after grafting may be relative to the vascularization of the dermal substitute." @default.
- W2385148624 created "2016-06-24" @default.
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- W2385148624 date "2003-01-01" @default.
- W2385148624 modified "2023-09-25" @default.
- W2385148624 title "MECHANISM OF THE FIBROBLAST INDUCING THE VASCULARIZATION OF DERMAL SUBSTITUTE" @default.
- W2385148624 hasPublicationYear "2003" @default.
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