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- W2385152849 abstract "According to the 16S rRNA gene sequences specific for Ehrlichiae chaffeensis,a pair of primers and one TaqMan-MGB probe were designed.The real-time quantitative polymerase chain reaction was developed with the primers,probe,and the template (16S rRNA gene DNA fragment of E.chaffeensis). The sensitivity of this real-time quantitative PCR was about 30 times higher than that of the nested PCR used to detect homologous DNA.By this quantitative real-time PCR,the DNA sample of E.chaffeensis was positively detected but not other rickettsial or bacterial DNA samples and the variation coefficients of intra- and inter-assay reproducibility were 0.2%～2.0%.The results show that the method is useful for detection of tiny DNA of E.chaffeensis in various samples." @default.
- W2385152849 created "2016-06-24" @default.
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- W2385152849 date "2006-01-01" @default.
- W2385152849 modified "2023-09-25" @default.
- W2385152849 title "DEVELOPMENT OF A QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION ASSAY SPECIFIC FOR EHRLICHIAE CHAFFEENSIS" @default.
- W2385152849 hasPublicationYear "2006" @default.
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