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- W2385173683 abstract "The purified TSO45W-4BX antigen was used to immunize BALB/C mice with Ferund′s complete and incomplete adjuvant.After immunization for three times and the booster injection via tail vein mouse,the SP2/0 cell fusion was performed with 50 % PEG-4000.After screening with TSO45W-4BX antigen and GST double system indirect ELISA,18 TSO45W-4BX antigen positive hybridoma cells were obtained.Four of them were further subcloned for five times with limited dilution method and at last four monoclonal cell lines were gained.After vaccination of mouse to gain corresponding ascites,it was found that the antibody belonged to the IgG1 class,with light chain was κ type by colloidal gold labeled paper identified,the stability of the ascites was tested and chromosome typing of the hybridoma cell was performed,which proved that the monoclonal antibody could satisfy the requirement of the conventional experiment." @default.
- W2385173683 created "2016-06-24" @default.
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- W2385173683 date "2008-01-01" @default.
- W2385173683 modified "2023-09-26" @default.
- W2385173683 title "Development and characterization of monoclonal antibody against 45W-4BX of oncosphere of Taenia solium antigen" @default.
- W2385173683 hasPublicationYear "2008" @default.
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