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- W2385188093 abstract "[Objective] The aim was to screen the optimum method for decreasing the contamination rate of tissue culture for Zantedeschia antedeschia in initial culture.[Method]With the budding annual bulbet of red and yellow Zantedeschia as the cultured materials,4 factors of bath temperature,surface disinfection time,kinds of surface disinfectant and preservative were selected for orthogonal design and the contamination rate and browning rate of every combination were calculated after the bulbets were cultured for 10 d.[Result] The 4 selected factors all had very significant influence on the contamination rate(P0.01) and among them,the bath temperature,surface disinfection time and kinds of surface disinfectant had significant influence on the contamination rate(P0.05).From the signal factor,the contamination rate was lower when the bath temperature was 40 ℃,and when the bulbets were treated by 0.1% mercuric chloride,or treated without preservative.The browning rate was lower when the bath temperature was 45 ℃ and it could get better effect of decreasing the browning rate when the surface disinfection time of the treatment was short.[Conclusion]The contamination rate was lowest under the condition of the bath temperature of 40 ℃,treating the bulbets by 0.1% mercuric chloride treating for 20 min and the medium without preservative." @default.
- W2385188093 created "2016-06-24" @default.
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- W2385188093 date "2008-01-01" @default.
- W2385188093 modified "2023-09-24" @default.
- W2385188093 title "Screening on the Methods for Decreasing Contamination Rate of Tissue Culture for Zantedeschia antedeschia in Initial Culture" @default.
- W2385188093 hasPublicationYear "2008" @default.
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