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- W2385193075 abstract "Objective To detect the transcriptional activity of CK13 gene 5'flanking region in various cells, and to further understand the relationship between the tissue-specific expression, abnormal expression of CK13 gene in the epithelial carcinomas, and the transcriptional activity of CK13 gene 5'flanking region. Methods The CAT system was used to assay the effects of CK13 gene 5' flanking region on its transcription. The clone of pCAT-enhancer with CK13 gene 5' flanking region was constructed and transferred to various cells with the help of lipofectin. We detected the instant CAT expression and investigated the transcriptional activity of CK13 gene 5' flanking region in various cells. In the meantime, the CK13 gene expressions of these cells were detected using northern-blot and were investigated and between the 5' flanking region transcriptional activity and tissue-specific expression of the CK13 gene. Results The transcriptional activity of CK13 gene 5' flanking region was different in various cells. The CAT of the cell which didnot express CK13 gene was not detected, but that of the cell which expressed CK13 gene was very high. Conclusion Tissue-specific expression in different cells and abnormal expression in the epithelial carcinomas of CK13 gene were related to the 5' flanking region transcriptional activity of CK13 gene." @default.
- W2385193075 created "2016-06-24" @default.
- W2385193075 creator A5072158048 @default.
- W2385193075 date "2002-01-01" @default.
- W2385193075 modified "2023-09-23" @default.
- W2385193075 title "Tissue-specific expression of CK13 gene and the transcriptional activity of CK13 gene 5' flanking region" @default.
- W2385193075 hasPublicationYear "2002" @default.
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