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- W2385207859 abstract "Objective To clone cDNA of hypoxia inducible factor-1α(HIF-1α) and have a further study on its effect in gene therapy of tumor.Methods Total RNA was extracted from healthy adults' peripheral blood,and then carried on RT-PCR by two steps using specific primers.HIF-1α gene was constructed by gene cloning technique.Results There was a distinct and special amplified strip,about 2500bp,by agarose gel electrophoresis using the production of RT-PCR.It was identified to be human HIF-1α by enzyme digestion.Conclusion HIF-1α cDNA can be cloned successfully and provide the evidence for further study." @default.
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- W2385207859 date "2007-01-01" @default.
- W2385207859 modified "2023-09-24" @default.
- W2385207859 title "Gene cloning of hypoxia inducible factor-1α" @default.
- W2385207859 hasPublicationYear "2007" @default.
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