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- W2385241304 abstract "Cinnamoyl Co-A reductase is a key enzyme in lignin synthesis pathway. A CCR gene was cloned from the immature stem of Eucalyptus urophylla clone GLU4 and named EuCCR by using specific primers based on the highly conserved sequences of plant CCR. The g DNA and cDNA sequence of EuCCR were 2918 bp and 1045 bp respectively, which CDS encodes 336 amino acid residues. Eu CCR had more than 96% sequence homology with Eucalyptus that had been logged in Gen Bank, and its homology with Angophora and Corymbia were over 85%. EuCCR encoding sequence was analyzed, the result shows it has an entire FR_SDR_e domain, a NADP binding site and a substrate binding site. The homology with Theobroma cacao and others were over 84%. The physicochemical property, structure of Eu CCR and its phylogenetic analysis were analyzed by using bioinformatics tools and MEGA. The SDS-PAGE analysis showed that EuCCR was transformed into pQE30/M15 system and fusion protein with molecular weighting about 39 kD was successfully expressed in transformant. The cloning and expression of EuCCR gene provided some effective resources for enzymology research and further transgenic research." @default.
- W2385241304 created "2016-06-24" @default.
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- W2385241304 date "2014-01-01" @default.
- W2385241304 modified "2023-09-24" @default.
- W2385241304 title "Cloning and prokaryotic expression of cinnamoyl Co-A reductase of Eucalyptus urophylla clone GLU4" @default.
- W2385241304 hasPublicationYear "2014" @default.
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